引用本文:廖伟,刘大庆,冯承莲,等.不同生长阶段斑马鱼对Cu2+的毒性响应差异[J].环境科学研究,2020,33(3):626-633.
LIAO Wei,LIU Daqing,FENG Chenglian,et al.Difference in Toxicity Response of Zebrafish to Cu2+ at Different Life Stages[J].Research of Environmental Sciences,2020,33(3):626-633.]
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不同生长阶段斑马鱼对Cu2+的毒性响应差异
廖伟1,2,3, 刘大庆2, 冯承莲1,2, 金小伟4, 刘娜2, 白英臣1,2, 吴代赦1
1. 南昌大学资源环境与化工学院, 鄱阳湖环境与资源利用教育部重点实验室, 江西 南昌 330031;2. 中国环境科学研究院, 环境基准与风险评估国家重点实验室, 北京 100012;3. 江西省灌溉试验中心站, 江西 南昌 330201;4. 中国环境监测总站, 北京 100012
摘要:
为探究鱼类不同生长阶段对污染物的毒性响应差异,以斑马鱼为试验生物,初步建立了一套实验室分阶段培养方法,通过培养时间确定斑马鱼的不同生长阶段,研究了标准稀释水和模拟自来水条件下不同生长阶段斑马鱼对Cu2+的毒性响应差异,并利用物种敏感度分布法表达不同生长阶段斑马鱼对Cu2+的敏感性差异.结果表明:斑马鱼体长、体质量的变化均符合logistic增长方程(R2>0.97),斑马鱼全生命周期按日龄可细分为卵黄囊仔鱼期(1 d)、晚期仔鱼期(10 d)、稚鱼期(17 d)、早幼期(24 d)、幼鱼期(31 d)、发育期(60 d)、成熟期(90 d)、成鱼期(120 d)等8个不同生长阶段.标准稀释水条件下,Cu2+对不同生长阶段斑马鱼的96 h-LC50(96 h半致死浓度)分别为0.425 mg/L(1 d)、0.768 mg/L(10 d)、0.550 mg/L(17 d)、0.309 mg/L(24 d)、0.334 mg/L(31 d)、0.327 mg/L(60 d)、0.230 mg/L(90 d)和0.180 mg/L(120 d),随着鱼龄的增加,Cu2+对斑马鱼的毒性逐渐增大;模拟自来水条件下,Cu2+对不同生长阶段斑马鱼的96 h-LC50分别为0.377 mg/L(1 d)、0.438 mg/L(10 d)、0.366 mg/L(17 d)、0.201 mg/L(24 d)、0.206 mg/L(31 d)、0.189 mg/L(60 d)、0.167 mg/L(90 d)和0.144 mg/L(120 d),随着鱼龄的增加,Cu2+对斑马鱼的毒性逐渐增大.根据96 h-LC50得出不同生长阶段斑马鱼对Cu2+的敏感性顺序为120 d > 90 d > 24 d > 60 d > 31 d > 1 d > 17 d > 10 d,可见,斑马鱼成鱼期阶段对Cu2+较敏感,而成熟期和早幼期的敏感性次之,晚期仔鱼期最不敏感.研究显示,鉴于斑马鱼对Cu的物种敏感度的阶段性差异,采用24 d早幼期斑马鱼研究金属水质基准时既敏感又节约受试生物的培养成本,此外水环境参数也是水质基准研究需考虑的重要影响因素之一.
关键词:  斑马鱼  不同生命阶段  分阶段方法  急性毒性  物种敏感度分布  水质基准
DOI:10.13198/j.issn.1001-6929.2019.06.25
分类号:X171.5
基金项目:国家水体污染控制与治理科技重大专项(No.2017ZX07301005-001);国家自然科学基金项目(No.41503100)
Difference in Toxicity Response of Zebrafish to Cu2+ at Different Life Stages
LIAO Wei1,2,3, LIU Daqing2, FENG Chenglian1,2, JIN Xiaowei4, LIU Na2, BAI Yingchen1,2, WU Daishe1
1. Key Laboratory of Poyang Lake Environment and Resource Utilization, Ministry of Education, School of Environmental and Chemical Engineering, Nanchang University, Nanchang 330031, China;2. State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China;3. Jiangxi Irrigation Experiment Central Station, Nanchang 330201, China;4. China National Environmental Monitoring Center, Beijing 100012, China
Abstract:
In order to explore the different toxicity response of fish to pollutants at different growth stages, zebrafish was studied with a preliminary stage culture method in laboratory, and the growth stages of zebrafish were determined at different culture time. The difference in toxicity response of zebrafish to Cu2+ at different stages under standard dilution water and artificial tap water were studied. Additionally, the species sensitivity distribution method was used to evaluate the sensitivity differences of zebrafish to Cu2+ at different growth stages. The results showed that the increase of body length and body weight of zebrafish fitted the logistic growth equation (R2>0.97). The whole life of zebrafish could be divided into eight different growth stages, namely 1 d larvae stage, 10 d larvae stage, 17 d larvae stage, 24 d early-juvenile stage, 31 d juvenile stage, 60 d developmental stage, 90 d mature stage and 120 d adult stage. The 96 h-median lethal concentration (96 h-LC50) of Cu2+ to zebrafish was 0.425, 0.768, 0.550, 0.309, 0.334, 0.327, 0.230 and 0.180 mg/L for the 1, 10, 17, 24, 31, 60, 90 and 120 d zebrafish in standard diluted water, respectively. The toxicity of Cu2+ to zebrafish increased with the prolongation of fish age. The 96 h-LC50 of Cu2+ to zebrafish was 0.377, 0.438, 0.366, 0.201, 0.206, 0.189, 0.167 and 0.144 mg/L for 1, 10, 17, 24, 31, 60, 90 and 120 d zebrafish in simulated tap water, respectively. According to 96 h-LC50, the sensitivity of zebrafish to Cu2+ at different growth stages decreased in the order of 120 d > 90 d > 24 d > 60 d > 31 d > 1 d > 17 d > 10 d. The results showed that the adult zebrafish was the most sensitive stage, followed by the mature stage and early-juvenile stage, while the larvae stage was the insensitive stage. In view of the different Cu2+ toxicity to zebrafish at different life stage, taking the 24 d early-juvenile stage zebrafish to study water quality criteria of copper was both sensitive and economical. Furthermore, water environmental parameters will be the important factors for the study of water quality criteria.
Key words:  zebrafish  different life stages  phased methodology  acute toxicity  species sensitivity distribution  water quality criteria