引用本文:赵串串,侯文涛,温怀峰,等.外源脯氨酸对Cd胁迫下107杨光合与叶绿素荧光特性的影响[J].环境科学研究,2020,(1):192-199.
ZHAO Chuanchuan,HOU Wentao,WEN Huaifeng,et al.Effects of Exogenous Proline on Photosynthetic and Chlorophyll Fluorescence Characteristics of Populus×euramericana cv. ‘74/76’ under Cadmium Stress[J].Research of Environmental Sciences,2020,(1):192-199.]
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外源脯氨酸对Cd胁迫下107杨光合与叶绿素荧光特性的影响
赵串串, 侯文涛, 温怀峰, 汲绍文, 王雪, 余登举
陕西科技大学环境科学与工程学院, 陕西 西安 710021
摘要:
为揭示Cd胁迫下外源脯氨酸对107杨(Populus×euramericana cv.‘74/76’)光合与叶绿素荧光特性的影响,以一年生107杨苗木为试验材料,采用盆栽控水试验,研究了添加不同浓度(0、10和20 mmol/L)外源脯氨酸对Cd(0、100、200和300 mg/kg)胁迫下107杨叶片光合气体交换参数、光响应参数和叶绿素荧光参数的影响.结果表明:①Cd作为独立因子可显著影响107杨叶片的光合气体交换参数、光响应参数和叶绿素荧光参数,外源脯氨酸作为独立因子对这些参数无显著影响,Cd与脯氨酸的交互作用对这3类参数均有显著影响.②随着w(Cd)的增加,107杨叶片净光合速率(Pn)、蒸腾速率(Tr)、气孔导度(Gs)和水分利用效率(WUE)的变化趋势基本一致,均显著下降,而胞间CO2浓度(Ci)呈显著上升趋势.与单独Cd处理组相比,在w(Cd)为200 mg/kg处理下,添加外源c(脯氨酸)为10 mmol/L时苗木叶片的Pn显著提高123.12%;WUE、PSⅡ原初反应最大量子效率(Fv/Fm)、实际光化学量子效率(φPSⅡ)、光化学淬灭系数(qP)和表观光合电子传递速率(ETR)分别显著提高43.73%、6.52%、27.99%、28.96%和63.26%;而非光化学淬灭系数(NPQ)显著降低19.97%.研究显示,w(Cd)低于200 mg/kg时,外源添加脯氨酸处理可增强107杨的PSⅡ光能转换效率和光强利用效率,防止过剩光能对光合机构的伤害,从而提高植株抗逆性与适应性,且以c(脯氨酸)为10 mmol/L时的处理效果较好.
关键词:  脯氨酸  镉胁迫  光合作用  叶绿素荧光参数
DOI:10.13198/j.issn.1001-6929.2019.02.04
分类号:X53
基金项目:青海省黄土丘陵区公益林生态效益监测项目(No.210170051)
Effects of Exogenous Proline on Photosynthetic and Chlorophyll Fluorescence Characteristics of Populus×euramericana cv. ‘74/76’ under Cadmium Stress
ZHAO Chuanchuan, HOU Wentao, WEN Huaifeng, JI Shaowen, WANG Xue, YU Dengju
School of Environmental Science and Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China
Abstract:
To reveal the effects of the exogenous proline on photosynthetic and chlorophyll fluorescence characteristics of Populus×euramericana cv. ‘74/76’ under cadmium (Cd) stress, seedlings of Peuramericana cv. ‘74/76’ were used in a pot experiment to investigate the effects of spraying proline (0, 10 and 20 mmol/L concentrations) on photosynthetic gas exchange parameters, light response parameters and the chlorophyll fluorescence parameters in leaves of Peuramericana cv. ‘74/76’ at 0, 100, 200 and 300 mg/kg Cd. The results showed that:(1) As an independent factor, Cd significantly affected the photosynthetic gas exchange parameters, the light response parameters and the chlorophyll fluorescence parameters while the exogenous proline as an independent factor had no obvious effects on these three parameters. However, the interaction between Cd and the proline significantly affected all the three parameters. (2) With the increase of Cd concentration, the variation trend of the net photosynthetic rate (Pn), the transpiration rate (Tr), the stomatal conductance (Gs) and the water use efficiency (WUE) in leaves of Peuramericana cv. ‘74/76’ were basically similar and all of them were reduced obviously, while the intercellular CO2 concentration (Ci) showed a clear rising tendency. In addition, compared with the only Cd treatment group, the Pn of the seedlings applied by 10 mmol/L of the exogenous proline was remarkably increased by 123.12% under 200 mg/kg Cd stress. The WUE, PSⅡ maximal photochemistry efficiency (Fv/Fm), the actual photochemical quantum efficiency (φPSⅡ), the photochemical quenching coefficient (qP), and electron transfer rate (ETR) were all significantly increased by 43.73%, 6.52%, 27.99%, 28.96% and 63.26%, respectively. While the non-photochemical quenching coefficient (NPQ) decreased by 19.97%. In conclusion, when Cd concentration was less than 200 mg/kg, the exogenous proline treatment could enhance the PSⅡ light energy conversion efficiency and the light intensity utilization efficiency of Peuramericana cv. ‘74/76’, and further prevent the damage to the photosynthetic apparatus from the excess light energy, which mitigated inhibition of Cd stress on seedling growth and enhanced adaptability of Peuramericana cv. ‘74/76’ to Cd stress. The treatment with the concentration of 10 mmol/L proline was better.
Key words:  proline  cadmium stress  photosynthesis  chlorophyll fluorescence parameters